Overview of the GADP setup. (a) Schematic of the experimental setup, (b) GADP usage scenario during the experiment and container diagram of the three ASW volumes, (c) structural diagram and cross-section of the reactor chamber, (d) 3D structural diagram of the electrodes in the reactor chamber and the discharge principle. Courtesy of SIBET
Atmospheric pressure plasma technology has been demonstrated to have great potential for microbial inactivation due to its unique advantages, including no chemical residues, environmental friendliness, high treatment efficiency, low energy consumption, and easy to use.
Researchers at the Suzhou Institute of Biomedical Engineering and Technology (SIBET), Chinese Academy of Sciences, have proposed a strategy to sterilize marine aquaculture using gliding arc discharge plasma (GADP).
They independently developed an air-GADP device that functioned in seawater with different concentrations of Vibrio parahaemolyticus (VP).
VP is the most prevalent pathogen in intensive marine aquaculture worldwide, posing a serious threat to both aquaculture and public health safety. Currently, antibiotics are the main means of preventing and controlling VP infections in marine aquaculture. However, the large-scale and excessive use of antibiotics inevitably leads to problems such as antibiotic residues and bacterial resistance in seafood.
Optical emission spectroscopy (OES) diagnostics showed that GADP is rich in reactive species, including hydroxyl radicals, excited state oxygen atoms, singlet oxygen, and nitric oxide.
In a new study, SIBET researchers conducted a dose-effect study on the inactivation of VP in different volumes of artificial seawater using GADP. Results showed that GADP treatment completely inactivated VP at a volume of 5000 mL within 20 minutes.
The results of the study, titled “Efficacy of gliding arc discharge plasma in disinfecting artificial seawater contaminated with Vibrio parahaemolyticus,” have been published in the Journal of Hazardous Materials.
Inactivation effect of GADP on VP in 50 mL, 500 mL and 5000 mL of ASW with VP concentrations of (a) 1.0×103 CFU/mL, (b) 5.0×103 CFU/mL and (c) 10×103 CFU/mL. Courtesy of SIBET Potential bactericidal mechanism of GADP in artificial seawater. Courtesy of SIBET
Furthermore, the measurement data of pH, salinity, temperature and nitrate content before and after treatment indicate that GADP does not significantly affect the main water quality parameters of the artificial seawater.
The researchers investigated the potential bactericidal mechanisms of GADP in artificial seawater by examining hydroxyl radicals, hydrogen peroxide, redox potential, intracellular reactive oxygen species, and cell membrane permeability.
This study provides a viable solution against infection by the halophilic pathogen Vibrio parahaemolyticus and demonstrates the potential of GADP in seawater disinfection.
Further information: Meng-Ru Du et al., “Efficacy of gliding arc discharge plasma in disinfecting artificial seawater contaminated with Vibrio parahaemolyticus,” Journal of Hazardous Materials (2024). DOI: 10.1016/j.jhazmat.2024.135015
Courtesy of the Chinese Academy of Sciences
Source: Scientists propose gliding arc discharge plasma seawater disinfection technology for marine aquaculture (August 19, 2024) Retrieved August 19, 2024 from https://phys.org/news/2024-08-scientists-gliding-arc-discharge-plasma.html
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